A conundrum of r-protein stability: unbalanced stoichiometry of r-proteins during stationary phase in Escherichia coli - Supplementary materials
| Name | Size | Description |
|---|---|---|
| README.txt | 1.332Kb | README file |
| Figure_S1_Number_of_viable_cells_during_stationary_phase_culture.pdf | 35.70Kb | Figure S1 – Number of viable cells during stationary phase culture |
| Figure_S2_Ribosome_analysis_of_stationary_phase_cells_using_sucrose_gradient_centrifugation.pdf | 187.7Kb | Figure S2 – Ribosome analysis of stationary phase cells using sucrose gradient centrifugation |
| Figure_S3_Presence_of_100S_particles_in_stationary_phase_using_sucrose_gradient_centrifugation.pdf | 49.63Kb | Figure S3 – Presence of 100S particles in stationary phase using sucrose gradient centrifugation |
| Figure_S4_Total_RNA_content_in_cells_decreases_during_the_stationary_phase.pdf | 55.86Kb | Figure S4 – Total RNA content in cells decreases during the stationary phase |
| Figure_S5_16S_and_23S_rRNA_quantification_in_total_RNA.pdf | 88.12Kb | Figure S5 – 16S and 23S rRNA quantification in total RNA |
| Figure_S6_The_r_protein_quantity_in_the_70S_ribosomes_does_not_change_during_the_stationary_phase.pdf | 55.19Kb | Figure S6 – The r-protein quantity in the 70S ribosomes does not change during the stationary phase |
| Figure_S7_bL17_uL22_and_bS6_bind_non_specifically_to_ribosomes_during_isolation.pdf | 33.82Kb | Figure S7 – bL17 uL22 and bS6 bind non specifically to ribosomes during isolation |
| Figure_S8_Individual_50S_r_proteins_H_M_ratio_changes_in_proteome_during_stationary_phase.pdf | 78.92Kb | Figure S8 – Individual 50S r-proteins HM ratio changes in proteome during stationary phase |
| Figure_S9_Individual_30S_r_proteins_H_M_ratio_changes_in_proteome_during_stationary_phase.pdf | 62.52Kb | Figure S9 – Individual 30S r-proteins HM ratio changes in proteome during stationary phase |
| Figure_S11_50S_subunits_r_protein_degradation_kinetics.pdf | 1.451Mb | Figure S11 – 50S subunits r-protein degradation kinetics |
| Figure_S12_30S_subunits_r_protein_degradation_kinetics.pdf | 1.023Mb | Figure S12 – 30S subunits r-protein degradation kinetics |
| Supplementary_methods_and_figure_legends.pdf | 868.9Kb | Supplementary methods and figure legends |
| Table_S1_Statistical_values_of_bonferroni's_multiple_comparisons_test_of_the_ribosome_subunit_ratios_(Figure_2B)_dataset.pdf | 379.0Kb | Table S1 – Statistical values of bonferroni's multiple comparisons test of the ribosome subunit ratios (Figure 2B) dataset |
| Table_S2_Statistical_values_of_bonferroni's_multiple_comparisons_test_of_the_total_RNA_measurement_(Figure_3)_dataset.pdf | 377.9Kb | Table S2 – Statistical values of bonferroni's multiple comparisons test of the total RNA measurement (Figure 3) dataset |
| Table_S3_Statistical_values_of_bonferroni's_multiple_comparisons_test_of_r_protein_quantity_((L+H)M_ratio)_in_the_70S_ribosomes_from_stationary_phase_(Figure_S4)_dataset.pdf | 603.0Kb | Table S3 – Statistical values of bonferroni's multiple comparisons test of r-protein quantity ((L+H)M ratio) in the 70S ribosomes from stationary phase (Figure S4) dataset |
| Table_S4_Statistical_values_of_bonferroni's_multiple_comparisons_test_of_the_r_protein_quantity_((L+H)M_ratio)_in_the_day_1_proteome_(Figure_5A)_dataset.pdf | 552.8Kb | Table S4 – Statistical values of bonferroni's multiple comparisons test of the r-protein quantity ((L+H)M ratio) in the day 1 proteome (Figure 5A) dataset |
| Table_S5_Statistical_values_of_bonferroni's_multiple_comparisons_test_of_the_r_protein_quantity_(HM_ratio)_in_the_total_proteome_(Figure_5B)_dataset.pdf | 597.1Kb | Table S5 – Statistical values of bonferroni's multiple comparisons test of the r-protein quantity (HM ratio) in the total proteome (Figure 5B) dataset |
| Table_S6_Statistical_values_of_non_linear_reggression_modelling_of_the_r_protein_quantity_(HM_ratio)_in_stationary_phase_proteome_dataset.pdf | 529.5Kb | Table S6 – Statistical values of non linear regression modelling of the r-protein quantity (HM ratio) in stationary phase proteome dataset |
| Table_S7_R_proteins_free_pool_size_comparison_in_stationary_phase_(day_8)_and_exponential_growth_(Chen_et_al_2012).pdf | 692.8Kb | Table S7 – R-proteins free pool size comparison in stationary phase (day 8) and exponential growth (Chen et al 2012) |
| Table_S8_Amount_of_lysate_in_A260_units_applied_to_sucrose_gradients.pdf | 398.4Kb | Table S8 – Amount of lysate in A260 units applied to sucrose gradients |
| Table_S9_R_protein_unique_peptide_count_in_r_protein_quantity_in_the_70S_ribosomes_from_stationary_phase_(Figure_S4)_dataset.pdf | 428.3Kb | Table S9 – R-protein unique peptide count in r-protein quantity in the 70S ribosomes from stationary phase (Figure S4) dataset |
| Table_S10_R_protein_unique_peptide_count_in_r_protein_quantity_in_the_stationary_phase_proteome_(Figure_5B)_dataset.pdf | 424.4Kb | Table S10 – R-protein unique peptide count in r-protein quantity in the stationary phase proteome (Figure 5B) dataset |
Abstract
Here we show that a specific set of r-proteins are rapidly degraded after release from the rRNA. The degradation of r-proteins is an intriguing new aspect of r-protein metabolism in bacteria.
The dataset represents collection of supplementary materials used in the manuscript "A conundrum of r-protein stability: unbalanced stoichiometry of r-proteins during stationary phase in Escherichia coli".
Keyword
Ribosome; ProteomicsItem type
info:eu-repo/semantics/dataset; ImageCollections
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Except where otherwise noted, this item's license is described as info:eu-repo/semantics/openAccess