UNDER REVIEW: A conundrum of r-protein stability: unbalanced stoichiometry of r-proteins during stationary phase in Escherichia coli - Supplementary materials

Reier, Kaspar; Lahtvee, Petri-Jaan; Liiv, Aivar; Remme, Jaanus
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Figure S1 – Ribosome analysis of stationary phase cells using sucrose gradient centrifugation.pdf177.1KbSupplementary figure S1 – Ribosome analysis of stationary phase cells using sucrose gradient centrifugation
Figure S2 – Presence of 100S particles in stationary phase using sucrose gradient centrifugation.pdf50.84KbSupplementary figure S2 – Presence of 100S particles in stationary phase using sucrose gradient centrifugation
Figure S3 – Total RNA content in cells decreases during the stationary phase.pdf56.69KbSupplementary figure S3 – Total RNA content in cells decreases during the stationary phase
Figure S4 – The r-protein quantity in the 70S ribosomes does not change during the stationary phase.pdf54.77KbSupplementary figure S4 – The r-protein quantity in the 70S ribosomes does not change during the stationary phase
Figure S5 – bL17, uL22, and bS6 bind non-specifically to ribosomes during isolation.pdf33.39KbSupplementary figure S5 – bL17, uL22, and bS6 bind non-specifically to ribosomes during isolation
Figure S6 – Individual 50S r-proteins H-M ratio changes in proteome during stationary phase.pdf76.73KbSupplementary figure S6 – Individual 50S r-proteins H/M ratio changes in proteome during stationary phase
Figure S7 – Individual 30S r-proteins H-M ratio changes in proteome during stationary phase.pdf60.43KbSupplementary figure S7 – Individual 30S r-proteins H/M ratio changes in proteome during stationary phase
Figure S8 – R-proteins L-M ratio in the proteome during the stationary phase.pdf34.04KbSupplementary figure S8 – R-proteins L/M ratio in the proteome during the stationary phase
Figure S9 – 50S subunits r-protein degradation kinetics.pdf1.450MbSupplementary figure S9 – 50S subunits r-protein degradation kinetics
Figure S10 – 30S subunits r-protein degradation kinetics.pdf1.022MbSupplementary figure S10 – 30S subunits r-protein degradation kinetics
Supplementary figure legends.pdf135.4KbSupplementary figure legends
Table S1 - Statistical values of bonferroni's multiple comparisons test of the ribosome subunit ratios (Figure 2B) dataset.pdf379.5KbStatistical values of bonferroni's multiple comparisons test of the ribosome subunit ratios
Table S2 - Statistical values of bonferroni's multiple comparisons test of the total RNA measurement (Figure 3) dataset.pdf379.6KbStatistical values of bonferroni's multiple comparisons test of the total RNA measurement
Table S3 - Statistical values of bonferroni's multiple comparisons test of r-protein quantity ((L+H)M ratio) in the 70S ribosomes from stationary phase (Figu.pdf603.0KbStatistical values of bonferroni's multiple comparisons test of r-protein quantity ((L+H)M ratio) in the 70S ribosomes from stationary phase
Table S4 - Statistical values of bonferroni's multiple comparisons test of the r-protein quantity ((L+H)M ratio) in the day 1 proteome (Figure 5A) dataset.pdf552.8KbStatistical values of bonferroni's multiple comparisons test of the r-protein quantity ((L+H)M ratio) in the day 1 proteome
Table S5 - Statistical values of bonferroni's multiple comparisons test of the r-protein quantity (HM ratio) in the total proteome (Figure 5B) dataset.pdf597.0KbStatistical values of bonferroni's multiple comparisons test of the r-protein quantity (HM ratio) in the total proteome
Table S6 - Statistical values of non-linear reggression modelling of the r-protein quantity (HM ratio) in stationary phase proteome dataset.pdf529.5KbStatistical values of non-linear reggression modelling of the r-protein quantity (HM ratio) in stationary phase proteome
Table S7 - R-proteins free pool size comparison in stationary phase (day 8) and exponential growth (Chen et al. 2012).pdf692.8KbR-proteins free pool size comparison in stationary phase (day 8) and exponential growth
Table S8 - R-protein unique peptide count in r-protein quantity in the 70S ribosomes from stationary phase (Figure S4) dataset.pdf428.3KbR-protein unique peptide count in r-protein quantity in the 70S ribosomes from stationary phase
Table S9 - R-protein unique peptide count in r-protein quantity in the stationary phase proteome (Figure 5B) dataset.pdf424.5KbR-protein unique peptide count in r-protein quantity in the stationary phase proteome
README.txt1.332KbREADME file
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Date
2022
URI
https://datadoi.ee/handle/33/436
http://dx.doi.org/10.23673/re-310
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Abstract
Here we show that a specific set of r-proteins are rapidly degraded after release from the rRNA. The degradation of r-proteins is an intriguing new aspect of r-protein metabolism in bacteria. The dataset represents collection of supplementary materials used in the manuscript "A conundrum of r-protein stability: unbalanced stoichiometry of r-proteins during stationary phase in Escherichia coli".
Keyword
Ribosome; Proteomics
Item type
info:eu-repo/semantics/dataset; Image
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