Sequence data of HUMB collection 1.0 Authors: Reet Mändar, Tiiu Rööp University of Tartu, Estonia Corresponding author: Reet Mändar Contact: reet.mandar@ut.ee Human Microbiota Biobank (HUMB) at University of Tartu, Estonia, was established to support and promote research activities in the field of human microbiota. The collection contains more than 20,000 bacterial strains, both lactic acid bacteria and opportunistic bacteria. Webpage of the HUMB collection (hosted by the webpage of Estonian Electronic Microbial dataBase): http://eemb.ut.ee/eng Catalogue of the HUMB collection: https://eemb.ut.ee/humb The dataset contains sequence data of a portion of the strains. The data have been obtained using 16S sequencing of the DNA extracted from the strains. The dataset includes the following columns: HUMB Nr – number of strain in HUMB catalogue Strain Nr – number of strain originating from lab testings Organism – name of microorganism Genbank Accession Number – unique identifier assigned to genetic sequences in the GenBank database.