*** Clostridium butyricum T2F3 specific IgA and IgG antibody response detection using immunoblot***
Authors: Celeste Peterson1, Aili Tagoma1, Kristi Alnek1, Anu Bärenson1,2, Tamara Vorobjova1, Ija Talja1, Helis Janson1, Anne Kirss3, Siiri Kõljalg4,5, Aki Sinkkonen6, Marja Irmeli Roslund6, HEDIMED Investigator group and Raivo Uibo1

1 Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu 50411, Estonia.
2 Children’s Clinic, Tartu University Hospital, Tartu, Estonia.
3 Women’s Clinic, Tartu University Hospital, Tartu, Estonia.
4 Department of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
5 Laboratory of Clinical Microbiology, United Laboratories, Tartu University Hospital, Tartu, Estonia. 
6 Horticulture Technologies, Natural Resources Institute Finland, Turku, Finland.

Corresponding author: Celeste Peterson
email: celeste.peterson@ut.ee


***General information***
This collection of figures contains data from immunoblot analysis used for detection of Clostridium butyricum specific IgA and IgG antibodies in children aged 1–6 years. 
Antibody binding against bacterial proteins is shown by bands (red staining) on polyvinylidene fluoride membrane.


***Methodology***
100 ?g of proteins (originating from Clostridium butyricum) were loaded on a gradient gel. Separated antigens were transferred to a polyvinylidene fluoride membrane (0.45 ?m pore size). All Blue 10-250 kDa molecular weight markers (Bio-Rad, USA) were used. The tested sample was diluted 1/50 for IgA and 1/100 for IgG detection. Horseradish peroxidase-conjugated rabbit polyclonal anti-human IgA or IgG antibodies (Invitrogen, USA) were applied at 1/2000 dilution. 


***Description of the data in the data set***
Data specific information: IgA reactions are marked with * and IgG reactions are left with blank. Positive control strip with groEL antibody is marked with + and negative control with –.

***Sharing and Access information***
This data is made public for other researchers to use in their own work under CC-BY-SA.