Dataset title: UT-GPCR001 microscopy of ligand binding to M4 muscarinic receptor in live CHO-K1-hM4 cells Authors: Tahk, Maris-Johanna; Torp, Jane; Ali, Mohammed A.S.; Fishman, Dmytro; Parts, Leopold; Grätz, Lukas; Müller, Christoph; Keller, Max; Veiksina, Santa; Laasfeld, Tõnis; Rinken, Ago Contact information: laasfeld@ut.ee; ago.rinken@ut.ee Chair of Bioorganic chemistry, Institute of Chemistry, University of Tartu General dataset description: The dataset was gathered as a part of Tahk MJ, Torp J, Ali MA, Fishman D, Parts L, Grätz L, Müller C, Keller M, Veiksina S, Laasfeld T, Rinken A. Live-cell microscopy or fluorescence anisotropy with budded baculoviruses-which way to go with measuring ligand binding to M4 muscarinic receptors?. bioRxiv. 2021 Jan 1. https://doi.org/10.1101/2021.12.22.473643 The dataset consists of multiple zip files each of which corresponds to a single microscopy experiment. Each experiment is divided into three folders (experimental data folder, cell segmentation masks obtained via Ilastik random forest model RF-BF-2 analysis and cell segmentation masks obtained via U-Net3 based deep-learning model U-Net3-BF-1 analysis) Each experiment folder is named as Date_Time_Receptor-source_Ligand_experiment type Subfolders in each experiment folder are named as follows Raw data folder: _